Research Article

Stability determination of candidate reference genes in cucumber plants subjected to stresses from Phytophthora melonis

Tingquan Wu*, Rui Wang, Hu Du, Qingmin Jin, Yujuan Zhong, , Chunpeng Yao, Xiaomei Xu and Yu’e Lin

Published: 15 April, 2019 | Volume 3 - Issue 1 | Pages: 028-035

Stable reference genes are indispensable for ensuring the fidelity of determined gene expression levels. However, the expression levels of reference genes are unable to remain constant under all possible experimental conditions. Therefore, the stability determination of reference genes is necessary in an experimental system set. In the preset study, the stability of nine cucumber candidate reference genes (CsACT, CsUBQ, CsEF1α, CsCYP, CsαTU, CsCACS, CsTIP41, CsYSL8 and CsHEL) subjected to stresses from Phytophthora melonis(P. melonis) were determined using four different analysis methods, including Delta Ct, BestKeeper, NormFinder and GeNorm. The study results revealed that CsUBQ and CsCYP were the most stable genes suitable as internal control in cucumber plants under attack by P. melonis condition.

Read Full Article HTML DOI: 10.29328/journal.jpsp.1001028 Cite this Article Read Full Article PDF


Cucumber; Reference gene; P. melonis; Gene stability


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