Unlocking Landrace Potential Through race-specific screening and Field-Level Resistance evaluation for Durum wheat (Triticum turgidum subsp. durum (Desf.) Stem Rust Resistance under Natural Epidemic
Landrace accession seedling and field evaluation for resistance screening.
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Abstract: Durum wheat (Triticum turgidum subsp. durum [Desf.]) Husn is a significant global food cereal. The stem rust caused by P. graminis f.sp. tritici can result in a yield loss of up to 100%. This study aimed to identify seedling-stage resistance of durum wheat landrace accessions to prevailing races (TTKSK, TKTTF, TRTTF, and JRCQC) of the pathogen and evaluate their performance under field conditions. A total of 34 landrace accessions were tested under controlled greenhouse and field conditions. Seedlings were inoculated at the Ambo Agricultural Research Center and assessed using the 0–4 scale. Selected accessions were further evaluated in the field at a hotspot location during the main growing season. Seedling evaluation results showed variability in genotype responses for the prevailing races. Fourteen landrace accessions (TD7226, TD7227, TD7365, TD8489, TD3750, TD3751, TD3762, TD3764, TD8217, TD8218, TD8777, TD6309, TD6984, and TD8507) exhibited resistance (IT of 2 or below) types of infections to all four races. Some accessions displayed a vulnerable response with score of 3- to 3.). A highly significant (p<0.001) correlation was observed between disease, plant parameters, and yield. Based on the field FRS (<30s), CI (<20) and AUDPC (30%) of the check variety, accessions TD3750, TD751, TD5917, and TD8778 exhibited high partial resistance. Identifying and using these landraces accessions can be beneficial in the development of durable resistance breeding strategies for novel resistant wheat varieties. However, the effectiveness of this landrace requires further molecular investigation to identify the resistance source.
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Copyright (c) 2025 Melkie W, et al.
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Zelalem Bekeko, School of Plant Science, Haramaya University, P.O. Box 138. Dire Dawa, Ethiopia
Zelalem Bekeko is an haramaya university instructor and former dean of College of Agriculture and Environmental Science, specializing in plant pathology, particularly maize pathology. He holds a Ph.D. in Plant Pathology from Haramaya University, where he is also an Associate Professor. His research focuses on disease management, crop productivity, and the impact of agricultural practices on crop yields, with a particular emphasis on Ethiopian agriculture.
1. FAO STAT. Crops and Livestock Products. FAO Statistics. 2022. Available from: https://www.fao.org/faostat/en/#data/QC.
2. Central Statistical Agency. Agricultural Sample Survey 2020-21. Volume I: Report on Area and Production of Major Crops. Addis Ababa. 2021. Available from: https://ess.gov.et/wp-content/uploads/2013/09/2013EC.LAND-UTILIZATION-2021.pdf
3. Belayneh A, LVFWOF. Virulence analysis of Puccinia graminis f. sp. tritici populations in Ethiopia with special consideration of Ug99. Plant Pathol. 2009; 58(2):362–9. Available from: https://doi.org/10.1111/j.1365-3059.2008.01976.x
4. Effect of lime and vermicompost amendments on selected soil properties and wheat (Triticum aestivum L.) productivity on Nitisols of Negasa area, East Wollega Zone, Ethiopia. Available from: http://ir.haramaya.edu.et/hru/handle/123456789/2060
5. FAO. Crop Prospects and Food Situation - Quarterly Global Report No. 1, March 2022. 2019.
6. Minot N, Warner J, Lemma S, Kasa L, Gashaw A, Rashid S. The wheat supply chain in Ethiopia: Patterns, trends, and policy options. Gates Open Res. 2019; 3(174). Available from: https://doi.org/10.21955/gatesopenres.1115226.1
7. Royo C. Durum wheat: A global perspective. Agronomy. 2016; 6(3):54.
8. FAO. The spread of damaging wheat rust continuous new races found in Europe, Africa, and Central Asia. FAO Report. 2017; 40
9. C. Relationship between stripe rust (Puccinia striiformis) and common wheat (Triticum aestivum) yield loss in the highlands of Bale, southeastern Ethiopia. Arch Phytopathol Plant Prot. 2009; 42(6):508–23. Available from: http://dx.doi.org/10.1080/03235400701191663
10. Kolmer A, Ordonez ME, Groth JV. The Rust Fungi. In: Encyclopedia of Life Sciences. 2009; 18. Available from: https://www.ars.usda.gov/ARSUserFiles/3094/rust_fungi.pdf
11. Ayele B, BH. Incidence and challenges of rusts in wheat production, in Bishaw et al. (Ed.), Containing the Menace of Wheat Rusts: Institutionalized Interventions and Impacts.
12. Embete F, BAKZ. Identification of sources of resistance to stem rust of wheat, Puccinia graminis f.sp. tritici. Report of completed research project from 1999-2004. 2005; Volume I: Pathology. Available from: PPRC, Ambo.
13. Hei NB. Evaluation of wheat cultivars for slow rusting resistance to leaf rust (Puccinia triticina Eriks) in Ethiopia. Afr J Plant Sci. 2017; 11(23):23–9. Available from: http://dx.doi.org/10.5897/AJPS2016.1450
14. Khan MH, Bukhari A, Dar ZA, Rizvi SM. Status and strategies in breeding for rust resistance in wheat. Agric Sci. 2013; 4(6):292–301. Available from: http://dx.doi.org/10.4236/as.2013.46042
15. Rehman AU, Sajjad M, Profile S, Khan S, Ahmad N. Prospects of wheat breeding for durable resistance against brown, yellow, and black rust fungi. Int J Agric Biol. 2013; 15:1209–20. Available from: http://dx.doi.org/10.13140/2.1.4219.7121
16. Admassu B, Lind V, Friedt W, Ordon F. Virulence analysis of Puccinia graminis f.sp. tritici populations in Ethiopia with special consideration of Ug99. Plant Pathol. 2009; 58(2):362–9. Available from: https://doi.org/10.1111/j.1365-3059.2008.01976.x
17. Roelfs AP, Saari RE, McNab A. Rust Diseases of Wheat: Concepts and Methods of Disease Management. Mexico: CIMMYT. 1992; 81.
18. Zemede Lemma A. Genetic erosion, drought tolerance, and genotype by environment interaction of durum wheat (Triticum turgidum L.var durum) in Ethiopia. 2019. Available from: https://www.scribd.com/document/638051333/Untitled
19. Denbel W, Badebo A. Valuable sources of resistance in the Ethiopian durum wheat landraces to UG33 and other stem rust races. Int J Agron Plant Prod. 2012; 3(6):191–5. Available from: https://www.cabidigitallibrary.org/doi/pdf/10.5555/20123384629
20. Oliver R. Achieving durable disease resistance in cereals. Burleigh Dodds Science Publishing; 2021. Available from: https://www.bspp.org.uk/achieving-durable-disease-resistance-in-cereals/
21. Tilahun Hadis L, Negash Gure T, Kassa Habtemariam D, Muche Abebile G, Yirga Belayineh F, Ayele Zerihun A. Evaluation of Wheat Genotypes for a Single Stem Rust Race TTTTF in Ethiopia. Biomed Stat Inform. 2021; 6(3):47. Available from: http://dx.doi.org/10.11648/j.bsi.20210603.12
22. Chen J, Ubhayasekera N, Dong Y, Singh J, Lian F, Bao C, et al. Loss of AvrSr50 by somatic exchange in stem rust leads to virulence for Sr50 resistance in wheat. Science. 2017; 358(6370):1607–1610. Available from: https://doi.org/10.1126/science.aao4810
23. Jin Y, Szabo LJ, Fetch TJr, Pretorius ZA, Njau P. Detection of virulence to resistance gene Sr36 within the TTKS race lineage of Puccinia graminis f.sp. tritici. Plant Dis. 2009; 93:367–70. Available from: https://doi.org/10.1094/PDIS-93-4-0367
24. Olivera P, Newcomb M, Szabo LJ, Rouse M, Johnson J, Gale S, et al. Phenotypic and Genotypic Characterization of Race TKTTF of Puccinia graminis f.sp. tritici that Caused a Wheat Stem Rust Epidemic in Southern Ethiopia. Phytopathology. 2015; 2013–4. Available from: https://doi.org/10.1094/PHYTO-11-14-0302-FI
25. Leta G, Belay G, Worku W. Nitrogen Fertilization Effects on Grain Quality of Durum Wheat (Triticum turgidum L. Var. Durum) Varieties in Central Ethiopia. J Agric Sci Belihuloya. 2013; 1(1):1–7.
26. Denbel W, Badebo A, Alemu T. Evaluation of Ethiopian Commercial Wheat Cultivars for Resistance to Stem Rust of Wheat Race UG99. Int J Agron Plant Prod. 2013; 4:15–24. Available from: https://www.cabidigitallibrary.org/doi/pdf/10.5555/20133059480
27. Roelfs AP, Singh RP, Saari EE. Rust Diseases of Wheat: Concepts and Methods of Disease Management. Mexico, DF: CIMMYT. 1992. Available from: https://www.scirp.org/reference/referencespapers?referenceid=52073
28. Peterson R, Campbell A, Hannah AE. A Diagrammatic Scale for Estimating Rust Intensity on Leaves and Stems of Cereals. Can J Res. 1948;26:490–500. Available from: https://doi.org/10.1139/cjr48c-033
29. Stakman EC, Stewart DM, Loegering WQ. Identification of Physiologic Races of Puccinia graminis var. tritici. Revised Edition. Miscellaneous Publication No. E-617. St. Paul, MN: U.S. Department of Agriculture, Agricultural Research Service. 1962; 53. Available from: https://www.cabidigitallibrary.org/doi/full/10.5555/19631101079
30. Roelfs AP. Epidemiology in North America. In: Diseases, Distribution, Epidemiology, and Control. Academic Press. 1985;403–34. Available from: https://www.sciencedirect.com/science/article/abs/pii/B9780121484026500213
31. Wheeler BEJ. An Introduction to Plant Diseases. 1969;374. Available from: https://www.cabidigitallibrary.org/doi/full/10.5555/19701604180
32. Wilcoxson, Skovmand B, Atif AH. Evaluation of Wheat Cultivars for Ability to Retard Development of Stem Rust. Ann Appl Biol. 1975; 80(3):275–81. Available from: https://doi.org/10.1111/j.1744-7348.1975.tb01633.x
33. Van der Plank JE. Plant Diseases. Elsevier Science Academic Press. 1963;349. Available from: https://books.google.co.in/books/about/Plant_Diseases.html?id=I6iWez_Rvq8C&redir_esc=y
34. Madden LV, Hughes G, Van Den Bosch F. The Study of Plant Disease Epidemics. American Phytopathological Society. 2007; No. 632.3 M33. Available from: https://doi.org/10.1094/9780890545058
35. Hailu E, Woldaeb G, Danbali W, Alemu W, Abebe T. Distribution of Stem Rust (Puccinia graminis f.sp. tritici) Races in Ethiopia. Adv Crop Sci Tech. 2015; 3(3). Available from: http://dx.doi.org/10.4172/2329-8863.1000173
36. Letta T. Seedling Resistance to Stem Rust (Puccinia graminis f.sp tritici) and Molecular Marker Analysis of Resistance Genes in Some Wheat Cultivars. Plant. 2018; 6(1):16. Available from: http://dx.doi.org/10.11648/j.plant.20180601.13
37. Getaneh Woldeab, EH, N. Bacha. Protocols for Race Analysis of Wheat Stem Rust (Puccinia graminis f.sp. tritici). EIAR, Ambo, Ethiopia. 2017;1-26.Available from: www.globalrust.org/race-manual/Ambo.
38. Dretzke B. Statistics with Microsoft Excel. Prentice Hall Press. 2008. Available from: https://dl.acm.org/doi/abs/10.5555/1481657
39. Mohammad MJ, Mazahreh N. Changes in Soil Fertility Parameters in Response to Irrigation of Forage Crops with Secondary Treated Wastewater. Commun Soil Sci Plant Anal. 2003; 34(9-10):1281–94. Available from: https://doi.org/10.1081/CSS-120020444
40. Admassu B, Lind V, Friedt W, Ordon F. Virulence Analysis of Puccinia graminis f.sp. tritici Populations in Ethiopia with Special Consideration of Ug99. Plant Pathol. 2009;58(2):362–9. Available from: https://doi.org/10.1111/j.1365-3059.2008.01976.x
41. Pathan AK, Park RF. Evaluation of seedling and adult plant resistance to stem rust in European wheat cultivars. Euphytica. 2007;155(1):87–105. Available from: https://link.springer.com/article/10.1007/s10681-006-9308-z
42. Jeger MJ, Viljanen-Rollinson SLH. The use of the area under the disease-progress curve (AUDPC) to assess quantitative disease resistance in crop cultivars. Theoretical and Applied Genetics. 2001;102:32–40. Available from: https://link.springer.com/article/10.1007/s001220051615
43. Singh RP, Hodson DP, Huerta-Espino J, Jin Y, Njau P, Wanyera R, et al. Will Stem Rust Destroy the World’s Wheat Crop? Advances in Agronomy. 2008;98:271–309. Available from: https://doi.org/10.1016/S0065-2113(08)00205-8
44. Basnet BR, Singh S, Lopez-Vera EE, Huerta-Espino J, Bhavani S, Jin Y, et al. Molecular mapping and validation of SrND643: A new wheat gene for resistance to the stem rust pathogen Ug99 race group. Phytopathology. 2015;105(4):470–476. Available from: https://doi.org/10.1094/PHYTO-01-14-0016-R
45. Yu LX, Barbier H, Rouse MN, Singh S, Singh RP, Bhavani S, et al. A consensus map for Ug99 stem rust resistance loci in wheat. Theoretical and Applied Genetics. 2014;127(7):1561–1581. Available from: https://doi.org/10.1007/s00122-014-2326-7
46. Liu W, Maccaferri M, Rynearson S, Letta T, Zegeye H, Tuberosa R, et al. Novel sources of stripe rust resistance identified by genome-wide association mapping in Ethiopian durum wheat (Triticum turgidum ssp. durum). Front Plant Sci. 2017;8:774. Available from: https://doi.org/10.3389/fpls.2017.00774
47. Khan MH, Bukhari A, Dar ZA, Rizvi SM. Status and strategies in breeding for rust resistance in wheat. Agricultural Sciences. 2017;4(06):292. Available from: http://dx.doi.org/10.4236/as.2013.46042
48. Safavi SA, Ahari AB, Afshari F, Arzanlou M. Slow rusting resistance in Iranian barley cultivars to Puccinia striiformis f. sp. hordei. J Plant Prot Res. 2013;53(1):5–11. Available from: https://doi.org/10.2478/jppr-2013-0001
49. Zhang H, Chen X. Advances in breeding for disease resistance in crops: The role of genomics. Front Plant Sci. 2017;8:1038. Available from: https://doi.org/10.3389/fpls.2017.01038
50. Hei N, Shimelis HA, Laing M, Admassu B. Assessment of Ethiopian wheat lines for slow rusting resistance to stem rust of wheat caused by Puccinia graminis f. sp. tritici. Journal of Phytopathology. 2015;163(5):353–363. Available from: https://doi.org/10.1111/jph.12329
51. Prasad P, Thakur R, Bhardwaj SC, Savadi S, Gangwar OP, Lata C, et al. Virulence and genetic analysis of Puccinia graminis tritici in the Indian sub-continent from 2016 to 2022 and evaluation of wheat varieties for stem rust resistance. Front Plant Sci. 2023;14. Available from: https://doi.org/10.3389/fpls.2023.1196808
52. Singh M, Lakhran L, Kumar S, Kumar N, Prajapati S. Breeding approaches for disease resistance in crop plants: A review. Ann Clin Lab Sci. 2021;4(2):1022. Available from: https://meddocsonline.org/annals-of-biotechnology/breeding-approaches-for-disease-resistance-in-crop-plants-a-review.html
53. Periyannan S, Milne RJ, Figueroa M, Lagudah ES, Dodds PN. An overview of genetic rust resistance: From broad to specific mechanisms. PLoS Pathog. 2017;13(1):e1006380. Available from: https://doi.org/10.1371/journal.ppat.1006380
54. Dinglasan E, Periyannan S, Hickey LT. Harnessing adult-plant resistance genes to deploy durable disease resistance in crops. Essays in Biochemistry. 2022;66:571–580. Available from: https://doi.org/10.1042/EBC20210096
55. Brown JKM. Durable resistance of crops to disease: A Darwinian perspective. Annu Rev Phytopathol. 2015;53:513–539. Available from: https://doi.org/10.1146/annurev-phyto-102313-045914
56. Ali S, Shah SJA, Khalil IH, Raman H, Maqbool K, Ullah W. Partial resistance to yellow rust in introduced winter wheat germplasm in the north of Pakistan. Aust J Crop Sci. 2009;3:37–43. Available from: https://www.researchgate.net/publication/26575918_Partial_resistance_to_yellow_rust_in_introduced_winter_wheat_germplasm_at_north_of_Pakistan
57. Draz IS, Abou-Elseoud MS, Kamara AEM, Alaa-Eldein OAE, El-Bebany AF. Screening of wheat genotypes for leaf rust resistance along with grain yield. Ann Agric Sci. 2015;60(1):29–39. Available from: https://doi.org/10.1016/j.aoas.2015.01.001
58. Mitiku M, Bacha Hei N, Abera M. Characterization of slow rusting resistance against stem rust (Puccinia graminis f. sp. tritici) in selected bread wheat cultivars of Ethiopia. Adv Crop Sci Technol. 2018;6(5):389. Available from: http://dx.doi.org/10.4172/2329-8863.1000389
59. Shikha K, Chand R, Mishra NK, Singh S, Sayiprathap BR, Nair RM, et al. Components of slow disease development: A key to enhance resistance in crops. CABI Agric Biosci. 2024;5(81). Available from: https://doi.org/10.1186/s43170-024-00293-4
60. Chu B, Yang L, Wang C, Gu Y, Yuan K, Wang R, et al. Improved evaluation of wheat cultivars (lines) on resistance to Puccinia striiformis f. sp. Tritici using molecular disease index. Plant Dis. 2019;103(6):1206–1212. Available from: https://doi.org/10.1094/PDIS-07-18-1158-RE
61. Wang ZL, Li LH, He ZH, Duan XY, Zhou YL. Seedling and adult plant resistance to powdery mildew in Chinese bread wheat cultivars and lines. Plant Dis. 2005;89:457–463. Available from: https://doi.org/10.1094/PD-89-0457
62. Sakr N. In vitro quantitative resistance components in wheat plants to Fusarium head blight. Open Agric J. 201927;13(1):9–18. Available from: http://dx.doi.org/10.2174/1874331501913010009
63. Devi HM, Mahapatra S, Das S. Assessment of yield loss of wheat caused by spot blotch using a regression model. Indian Phytopathol. 2018;71:291–294. Available from: https://doi.org/10.1007/s42360-018-0036-9
64. Parlevliet JE. Partial resistance of plants to fungal pathogens: Partial resistance, quantitative resistance, or slow rusting. Euphytica. 1988;38:1–11.
65. Zhan J, Thrall PH, Papaïx J, Xie L, Burdon JJ. Playing on a pathogen’s weakness: Using evolution to guide sustainable plant disease control strategies. Annu Rev Phytopathol. 2015;53:19–43. Available from: https://doi.org/10.1146/annurev-phyto-080614-120040
66. Hei NB. Evaluation of wheat cultivars for slow rusting resistance to leaf rust (Puccinia triticina Eriks) in Ethiopia. Afr J Plant Sci. 2017;11:23–29. Available from: http://dx.doi.org/10.5897/AJPS2016.1450
67. Assefa M, Sileshi F, Bacha N, et al. Seedling resistance of selected Ethiopian bread and durum wheat lines against dominant stem rust races. J Plant Pathol. 2019;101:115–120. Available from: https://doi.org/10.1007/s42161-018-0157-0
68. Pretorius ZA, Singh RP, Wagoire TS, Payne T. Detection of virulence to wheat stem rust resistance gene Sr31 in Puccinia graminis f. sp. tritici in Uganda. Plant Dis. 2000;84(2):203. Available from: https://doi.org/10.1094/PDIS.2000.84.2.203B
69. Singh RP, Hodson DP, Jin Y, Huerta-Espino J, Kinyua MG, Wanyera R, et al. Will stem rust destroy the world’s wheat crop? Adv Agron. 2008;98:271–309. Available from: https://doi.org/10.1016/S0065-2113(08)00205-8
70. Ashmawy MA, Orabey WM, Shahin AA. Effect of stem rust infection on grain yield and yield components of some wheat cultivars in Egypt. Int J Phytopathol. 2013;2(3):171–178. Available from: https://esciencepress.net/journals/index.php/phytopath/article/view/308/236
71. Bethenod O, Huber L, Slimi H. Photosynthetic response of wheat to stress induced by Puccinia recondita and post-infection drought. Photosynthetica. 2001;39:581–590. Available from: https://doi.org/10.1023/A:1015664314720
72. Robert C, Bancal MO, Ney B, Lannou C. Wheat leaf photosynthesis loss due to leaf rust, with respect to lesion development and leaf nitrogen status. New Phytol. 2005 Jan;165(1):227–241. Available from: https://doi.org/10.1111/j.1469-8137.2004.01237.x
73. Chen YE, Cui JM, Su YQ, Yuan S, Yuan M, Zhang HY. Influence of stripe rust infection on the photosynthetic characteristics and antioxidant system of susceptible and resistant wheat cultivars at the adult plant stage. Front Plant Sci. 2015 Sep 28;6. Available from: https://doi.org/10.3389/fpls.2015.00779
74. Fernando P. Water relations of faba bean uninfected and infected with rust: Effects on transpiration, leaf water potential, and root growth. In: Rust Fungi and Global Change. 2001.
75. Craigie JH. Stem Rust of Cereals. Ottawa, ON, Canada: Department of Agriculture. 1957. Available from: https://publications.gc.ca/site/eng/9.800794/publication.html
76. Rowell JB, Bushnell WR. The premature death of adult rusted wheat plants in relation to carbon dioxide evolution by the root system. Phytopathology. 1968;58:651–658. Available from: https://www.cabidigitallibrary.org/doi/full/10.5555/19681102666
77. Safavi SA. Sources of resistance in advanced barley lines to Puccinia striiformis f. sp. hordei. Adv Environ Biol. 2012;6(2):708–712. Available from: https://www.researchgate.net/publication/289217652_Sources_of_resistance_in_advanced_barley_lines_to_Puccinia_striiformis_f_sp_hordei
78. Safavi SA, Afshari F. Identification of resistance to Puccinia striiformis f. sp. tritici in some elite wheat lines. J Crop Prot. 2012. Available from: https://www.researchgate.net/publication/303605019_Identification_of_resistance_to_Puccinia_striiformis_f_sp_tritici_in_some_elite_wheat_lines
79. Safavi S, Afshari F. A seven-year assessment of resistance durability to yellow rust in some wheat cultivars in Ardabil province, Iran. J Crop Prot. 2017;6(3):409–421. Available from: https://jcp.modares.ac.ir/article-3-10332-en.html
80. Siricord C, O’Brien PA. MALDI-TOF mass spectrometry can be used for detection of pathogenic microorganisms in soil. Australas Plant Pathol. 2008;37:543–545. Available from: https://link.springer.com/article/10.1071/AP08052
81. Adila W, Terefe H, Bekele A. Common bacterial blight (Xanthomonas axonopodis pv. phaseoli) resistance reaction in common bean genotypes and their agronomic performances in Southern Ethiopia. J Crop Sci Biotechnol. 2021;24:387–400. Available from: https://doi.org/10.1007/s12892-021-00087-4
82. Phenotypic and molecular characterization of wheat for slow rusting resistance against Puccinia striiformis Westend. f.sp. tritici. J Phytopathol. 2010;158:393–402. Available from: https://doi.org/10.1111/j.1439-0434.2009.01631.x
83. Iqbal N, Eticha F, Khlestkina EK, Weidner A, Röder MS, Börner A. The use of simple sequence repeat (SSR) markers to identify and map alien segments carrying genes for effective resistance to leaf rust in bread wheat. Plant Genet Resour. 2007;5(2):100–103. Available from: https://doi.org/10.1017/S1479262107672311
84. Zegeye H, Rasheed A, Makdis F, Badebo A, Ogbonnaya FC. Genome-wide association mapping for seedling and adult plant resistance to stripe rust in synthetic hexaploid wheat. PLoS One. 2014;9:e105593. Available from: https://doi.org/10.1371/journal.pone.0105593
85. Merkuz A, Getachew A. Impact of wheat stem rust (Puccinia graminis f. sp. tritici) on yield and yield components of wheat in South Tigray, Northern Ethiopia. J Plant Pathol Microbiol. 2012;3:1–4.
86. Ayele B, Alemayehu G, Disasa T. Impact of stem rust on grain yield and yield components of bread wheat in South Eastern Ethiopia. Afr J Agric Res. 2020;15:264–270.
87. Lemessa F, Yaynu Hailu. Relationship of common rust (Puccinia sorghi Schw.) and yield of maize in South-western Ethiopia. East Afr J Sci. 2011;5:57–65.
88. Boersma JG. Impact of leaf rust on wheat yield: Genotype, environment, and management interactions. Crop Prot. 2015;67:127–139.
89. Shewaye Y, Zegaye H, Tadesse Z, Solomon T, Asnake D, Alemu G, et al. Impact of stem and yellow rusts on grain yield of bread wheat (Triticum aestivum L.) genotypes under rainfed conditions of Ethiopia. Int J Bioorganic Chem. 2021;6(1):7. Available from: http://dx.doi.org/10.11648/j.ijbc.20210601.13
90. Park RF. Stem rust of wheat in Australia. Aust J Agric Res. 1994;45(6):1–10.
91. Cheng P, X. M, Campbell KG. Stripe rust resistance in wheat: Genes, genetic mapping, and breeding applications. Front Plant Sci. 2022;13:832316.