Functional Quality of Brazilian Barley: How Temperature and Rainfall Modulate β-Glucan and Dietary Fiber Levels
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Abstract
The objective of this study was to evaluate the impact of genotype and growing environment on the β-glucan and dietary fiber (DF) content of whole grain barley cultivars grown in Brazil. Cultivars BRS 225, BRS 195, and MN 743 were cultivated during the 2008 and 2009 crop years across two distinct locations (Passo Fundo and Victor Graeff). Analytical quantification followed AACC protocols for moisture, protein, and fiber fractions, while β-glucan was determined using the McCleary enzymic method. Significant differences (p < 0.05) were identified among genotypes and environments regarding β-glucan, total dietary fiber (TDF), soluble dietary fiber (SDF), and insoluble dietary fiber (IDF). Environmental variables, specifically air temperature, insolation, and rainfall, played a decisive role in fiber accumulation. Higher air temperatures and increased insolation were positively correlated with β-glucan levels, whereas rainfall generally exerted a negative influence. The substantial variation observed within the same location across different years underscores the necessity of considering both genetic potential and specific climatic windows to optimize the functional and nutritional quality of Brazilian barley.
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American Association of Cereal Chemists. Approved methods of the AACC: methods 32-07, 44-01, 46-12, and 55-10. 8th ed. St. Paul (MN): AACC; 1990.
Canteri MG, Althaus RA, Virgens Filho JS, Giglioti EA, Godoy CV. SASM - Agri: sistema para análise e separação de médias em experimentos agrícolas pelos métodos Scott-Knott, Tukey e Duncan. Rev Bras Agrocomputação. 2001;1(2):18-24.
Slavin J, Marquart L, Jacobs D. Consumption of whole-grain foods and decreased risk of cancer: proposed mechanisms. Cereal Foods World. 2000;45(1):54-58
Aman P, Newman CW. Chemical composition of some different types of barley grown in Montana, USA. J Cereal Sci. 1986;4(2):133-141. Available from: https://doi.org/10.1016/S0733-5210(86)80016-5
Jackson GD, Berg RK, Kushnak GD, Blake TK, Yarrow GI. Nitrogen effects on yield, beta-glucan content, and other quality factors for oat and waxy hulless barley. Commun Soil Sci Plant Anal. 1994;25(17-18):3047-3055. Available from: https://doi.org/10.1080/00103629409369245
Jenkins DJ, Jenkins AL, Wolever TMS, Rao AV, Thompson LV. Fiber and starchy foods: gut function and implications in disease. Am J Gastroenterol. 1985;81(10):920-930
Cavallero A, Empilli S, Brighenti F, Stanca AM. High (1→3, 1→4) - β-glucan barley fractions in bread making and their effects on human glycemic response. J Cereal Sci. 2002;36(1):59-66. Available from: https://doi.org/10.1006/jcrs.2002.0454
Yalcin E, Celik S, Akar T, Sayim I, Koksel H. Effects of genotype and environment on β-glucan and dietary fiber contents of hull-less barleys grown in Turkey. Food Chem. 2007;101(1):171-176. Available from: https://doi.org/10.1016/j.foodchem.2006.01.010
Fastnaught CE, Berglund PT, Helm ET, Fox GJ. Genetic and environmental variation in beta-glucan content and quality parameters of barley for food. Crop Sci. 1996;36(4):941-946. Available from: https://doi.org/10.2135/cropsci1996.0011183X003600040015x
Önning G. Carbohydrates and the risk of cardiovascular disease. In: Biliaderis CG, Izydorczyk MS, editores. Functional food carbohydrates. Boca Raton: CRC Press, Taylor & Francis Group; 2007. p. 291-319. Available from:
INMET – Instituto Nacional de Meteorologia. Agrometeorologia [Internet]. Brasília: INMET; 2012. Available from: http://www.inmet.gov.br/portal/index.php?r=agrometeorologia/boletinsAgroclimatologicos
Khaleghdoust B, Esmaeilzadeh-Salestani K, Korge M, Alaru M, Möll K, Värnik R, et al. Barley and wheat beta-glucan content is influenced by weather, fertilization, and genotype. Front Sustain Food Syst. 2024;7:1326716. Available from: https://doi.org/10.3389/fsufs. 2023.1326716
Karaman M, Seydoşoğlu S, Vyhnánek T, Ali MA, Elshikh MS. Interpretation of yield and quality traits of barley (Hordeum vulgare L.) varieties by heatmap technique. J King Saud Univ Sci. 2024;36(6):103382. Available from: https://doi.org/10.1016/j.jksus.2024.103382
Baik BK, Ullrich SE. Barley for food: characteristics, improvement, and renewed interest. J Cereal Sci. 2008;48(2):233-242. Available from: https://doi.org/10.1016/j.jcs.2008.02.002
Slavin J. Dietary carbohydrates and risk of cancer. In: Biliaderis CG, Izydorczyk MS, editores. Functional food carbohydrates. Boca Raton: CRC Press, Taylor & Francis Group; 2007. p. 371-385.
Saastamoinen M, Hietaniemi V, Pihlava JM, Eurola M, Kontturi M, Tuuri H, et al. Beta-glucan contents of groats of different oat cultivars in official variety, in organic cultivation, and in nitrogen fertilization trials in Finland. Agric Food Sci. 2004;13(1-2):68-79. Available from: https://doi.org/10.23986/afsci. 5684
Behall KM, Scholfield DJ, Hallfrisch J. Diets containing barley significantly reduce lipids in mildly hypercholesterolemic men and women. Am J Clin Nutr. 2004;80(5):1185-1193. Available from: https://doi.org/10.1093/ajcn/80.5.1185
Rolim JM, Martins AC, da Rosa CP, Barbosa BS, Martinazzo EG, Pedó T, Aumonde TZ. Desempenho fisiológico e bioquímico de cultivares de cevada sob estresse por restrição hídrica em diferentes temperaturas. Res Soc Dev. 2023;12(7):e11212741738. Available from: https://doi.org/10.33448 /rsd-v12i7.41738
Izydorczyk MS, Dexter JE. Barley b-glucans and arabinoxylans: molecular structure, physicochemical properties, and uses in food products–a review. Food Res Int. 2008;41(9):850-868. Available from: Available from: https://doi.org/10.1016/j.foodres.2008.07.008
McIntosh GH, Whyte J, McArthur R, Nestel PJ. Barley and wheat foods: influence on plasma cholesterol concentrations in hypercholesterolemic men. Am J Clin Nutr. 1991;53(5):1205-1209. Available from: https://doi.org/10.1093/ajcn/53.5.1205
Madhujith T, Izydorczyk MS, Shahidi F. Antioxidant properties of pearled barley fractions. J Agric Food Chem. 2006;54(9):3283-3289. Available from: https://doi.org/10.1021/jf0527712
McCleary BV, Codd R. Measurement of (1→3), (1→4)-β-D-glucan in barley and oats: a streamlined enzymic procedure. J Sci Food Agric. 1991;55(2):303-312. Available from: https://doi.org/10.1002/jsfa.2740550215
Jacobs DR Jr, Marquart L, Slavin J, Kushi LH. Whole-grain intake and cancer: an expanded review and meta-analysis. Nutr Cancer. 1998;30(2):85-96. Available from: https://doi.org/10.1080/01635589809514647
Andersson AM, Armo E, Grangeon E, Fredriiksson H, Andersson R, Aman P. Molecular weight and structure units of (1→3) (1→4) – β-D glucans in dough and bread made from hull-less barley fractions. J Cereal Sci. 2004;40(3):194-204. Available from: https://doi.org/10.1016/j.jcs.2004.08.003
Aastrup S. The effect of rain on β-glucan content in barley grains. Carlsberg Res Commun. 1979;44(6):381-393. Available from: https://doi.org/10.1007/BF02906491
Ehrenbergerová J, Belcredi NB, Psota V, Hrstková P, Cerkal R, Newman CW. Changes caused by genotype and environmental conditions in beta-glucan content of spring barley for dietetically beneficial human nutrition. Plant Foods Hum Nutr. 2008;63(3):111-117. Available from: https://doi.org/10.1007/s11130-008-0079-0
McCleary BV, Glennie-Holmes M. Enzymic quantification of (1→3), (1→4)-β-D-glucan in barley and malt. J Inst Brew. 1985;91(5):285-295. Available from: https://doi.org/10.1002/j.2050-0416.1985.tb04345.x