Figure 4

The Effects of Pharmacological Carbonic Anhydrase Suppression on Defence Responses of Potato Leaves To Phytophthora Infestans

Magdalena Arasimowicz-Jelonek* and Jolanta Floryszak-Wieczorek

Published: 03 March, 2017 | Volume 1 - Issue 1 | Pages: 011-025

jpsp-aid1002-g004

Figure 4:

Nitric oxide generation in extract of avr inoculated potato (Solanum tuberosum L.) leaves at 3 hpi. a) NO assayed with the use of the electrochemical approach and NO-selective microelectrode; b) NO determined fluorimetrically with the use of fluorochrome CuFL;c) NO determined by chemiluminescence method with Sievers Nitric Oxide Analyzer NOA 280i. NO detection was performed after addition of plant extract (E) and following reagents: 100µM NO2¯, 250µM dorzolamide (DA), 0.1mM tungstate (TG) and 5 mM Nω-Nitro-L-arginine methyl ester hydrochloride (L-NAME). At least three independent experiments were conducted; arrows indicate the point of adding the reagent.

Read Full Article HTML DOI: 10.29328/journal.jpsp.1001002 Cite this Article Read Full Article PDF

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